Plant Tissue Culture
Telangana Board · Class 12 · Biology
Flashcards for Plant Tissue Culture — Telangana Board Class 12 Biology. Quick Q&A cards covering key concepts, definitions, and formulas.
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What is tissue culture and what is the basic principle it is based on?
Answer
Tissue culture is the laboratory technique of growing, culturing and maintaining cells, tissues and organs in vitro on an artificial nutrient medium. It is based on the principle of 'cellular totipote…
Who coined the term 'totipotency' and in which year? Define totipotency.
Answer
Morgan coined the term 'totipotency' in 1901. Totipotency describes the capacity of a cell to develop into a complete organism by regeneration. It means each plant cell has the potential to give rise …
List the six steps involved in plant tissue culture technique in the correct sequence.
Answer
1. Preparation of nutrient culture medium 2. Sterilization of the culture medium 3. Preparation of explant 4. Inoculation of explant 5. Incubation for growth 6. Acclimatization of plantlets and transf…
What is an explant? Give examples of plant parts that can be used as explants.
Answer
An explant is any part of a plant which is introduced to culture medium to grow into a full-fledged plant or organs in vitro. Examples include: axillary bud, leaf segments, stem segments, root tip, sh…
What components are included in the preparation of nutrient culture medium and what is the optimal pH range?
Answer
Nutrient culture medium contains: macro and micro nutrients, amino acids, vitamins, carbohydrates mixed in distilled water. Agar is added for solidification to provide support. The pH is adjusted to 5…
Distinguish between basal medium and complete medium in tissue culture.
Answer
Basal medium: Does not contain growth regulators. Used for germinating seeds, developing aseptic seedlings, or single callus cultures. Complete medium: Contains growth regulators (auxins, gibberellins…
Why is sterilization of culture medium necessary and how is it carried out?
Answer
Sterilization is necessary because the culture medium is rich in nutrients and attracts microorganisms that contaminate and spoil the medium. Sterilization is carried out in an autoclave (steam steril…
How are explants prepared and sterilized before inoculation?
Answer
Explants from garden or potted plants have microorganisms on their surface. They are cleaned with liquid detergent in running water, then treated with disinfectant solutions like sodium hypochlorite t…
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