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CBSE Class 12 Biotechnology — Important Questions 2026

Most important questions for CBSE Class 12 Biotechnology board exam 2026. Chapter-wise questions with marking scheme and expected topics.

Quick Summary

This page covers the most important questions for CBSE Class 12 Biotechnology board exam 2026, based on previous year question papers and expected topics. Focus on these for maximum marks.

How to Use Important Questions

  1. Start with high-weightage chapters — focus on chapters that carry the most marks in the exam.
  2. Practice writing answers — don't just read; write out answers within the time limit to build speed.
  3. Cross-reference with syllabus — ensure every chapter is covered by checking the syllabus page.
  4. Revise with notes — after practising, use revision notes for quick recap before the exam.

Chapter-Wise Important Questions — 13 Chapters

Focus on high-weightage chapters first. Each chapter below lists the types of questions frequently asked in CBSE board exams.

1

An Overview of Recombinant DNA Technology

64 questions
multiple choice1 marks

Which scientists discovered plasmids in 1952?

multiple choice1 marks

Which structure of DNA was proposed in 1953?

multiple choice1 marks

Which enzyme acts as scissors to cut molecules of DNA?

multiple choice1 marks

Which enzyme joins two DNA fragments?

+60 more questions — practice in Super Tutor

2

Host–Vector System

64 questions
multiple choice1 marks

What is an episome?

multiple choice1 marks

Which plasmid category provides drug resistance by encoding enzymes that deactivate antibiotics?

multiple choice1 marks

Which plasmid category produces colicins that kill other bacteria?

multiple choice1 marks

Which plasmid category contains transfer genes for pilus formation and plasmid transfer?

+60 more questions — practice in Super Tutor

3

Gene Cloning

72 questions
multiple choice1 marks

What is the role of reverse transcriptase in cDNA preparation?

multiple choice1 marks

Which molecule is used to break the peptidoglycan of bacterial cell walls during DNA isolation?

multiple choice1 marks

Which detergent is used for bacterial cell lysis by disrupting the lipid bilayer?

multiple choice1 marks

What type of detergent is CTAB?

+68 more questions — practice in Super Tutor

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Frequently Asked Questions

Where can I find CBSE Class 12 Biotechnology Important Questions?

This page provides important questions for CBSE Class 12 Biotechnology for the 2026 board exam. For chapter-by-chapter study help including summaries, quizzes, and flashcards, try Super Tutor.

Biotechnology is one of the scoring subjects in CBSE Class 12 if prepared well. Focus on understanding concepts, practising problems regularly, and revising key formulas. Most students who follow a structured study plan score above 80%.

Start by understanding the complete syllabus. Then focus on important questions from each chapter. Use revision notes for quick review before exams. Follow a study plan that covers all chapters with dedicated revision time.

The question paper distribution varies each year, but certain chapters consistently carry more marks. Check the marks distribution section above for chapter-wise weightage based on previous year patterns.

Browse Important Questions by Chapter

13 chapters available

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