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Chapter 6 of 13
Important Questions

Microbial Culture — Important Questions

CBSE · Class 12 · Biotechnology

63 important questions from Microbial Culture for CBSE Class 12 Biotechnology, with answers. Written for the board exams 2027.

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A comparison diagram showing Louis Pasteur's swan-neck flask experiment, illustrating how life does not arise spontaneously from non-living matter.
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63 Questions·
multiple choicemultiple correcttrue falseassertion reasoning

Important Questions from Microbial Culture

1multiple choice
1 marks

Which period is called the Golden Age of Microbiology?

Show answer

Late 1800s to early 1900s

The late 1800s to early 1900s are called the Golden Age of Microbiology because many disease-causing microorganisms were discovered then.

2multiple choice
1 marks

What medium did Louis Pasteur use in 1860 for microbial growth?

Show answer

A medium containing yeast, ash and ammonium salts

Pasteur used a medium containing yeast, ash and ammonium salts, which supplied nutrients needed for growth.

3multiple choice
1 marks

Why did Ferdinand Cohn refine Pasteur's medium?

Show answer

By varying the types of sugars to make a more versatile basal medium

Ferdinand Cohn refined Pasteur's medium by varying sugar types so that the medium became more versatile.

4multiple choice
1 marks

In 1881, why did Robert Koch consider using solid media?

Show answer

Broth media made isolation of pure cultures difficult

Koch recognised the difficulties of using broth media for isolation and therefore considered solid media.

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Frequently Asked Questions

What are the important topics in Microbial Culture for CBSE Class 12 Biotechnology?
Key topics in Microbial Culture include Historical Perspective, Nutritional Requirements and Types of Media, Sterilisation Methods, Pure Culture Techniques. Study these first, then practise questions on each for the CBSE Class 12 board exam.
How many important questions are there in Microbial Culture?
Super Tutor has 63 practice questions for Microbial Culture, including multiple choice, multiple correct, true false, assertion reasoning questions. A sample with answers is on this page.

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Content is aligned to the official syllabus. Refer to the board website for the latest curriculum.

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