Gene Cloning
CBSE · Class 12 · Biotechnology
Most important questions from Gene Cloning for CBSE Class 12 Biotechnology board exam 2026. MCQs, short answer, and long answer questions with marks.
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What is the role of reverse transcriptase in cDNA preparation?
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It makes cDNA from an RNA template
Reverse transcriptase is an RNA-directed DNA polymerase that synthesises cDNA using RNA as the template.
Which molecule is used to break the peptidoglycan of bacterial cell walls during DNA isolation?
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Lysozyme
Lysozyme digests peptidoglycan, the main component of the bacterial cell wall.
Which detergent is used for bacterial cell lysis by disrupting the lipid bilayer?
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SDS
SDS is used to lyse cell membranes by disrupting the lipid bilayer.
What type of detergent is CTAB?
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Cationic detergent
CTAB is used for plant DNA isolation and is a cationic detergent.
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Sources & Official References
- NCERT Official — ncert.nic.in
- CBSE Academic — cbseacademic.nic.in
- CBSE Official — cbse.gov.in
- National Education Policy 2020 — education.gov.in
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