Biotechnology — Important Questions
Maharashtra Board · Class 12 · Biology
43 important questions from Biotechnology for Maharashtra Board Class 12 Biology, with answers. Includes multiple choice and multiple correct questions.
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Important Questions from Biotechnology
Which techniques are core to modern biotechnology?
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Genetic engineering, Chemical engineering
Modern biotechnology is based on two core techniques: genetic engineering (manipulation of genetic material) and chemical engineering (maintaining sterile environment for production). Traditional fermentation and selective breeding are part of classical biotechnology.
Which characteristics make EcoRI recognition sequence a palindrome?
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Reads same in both directions, Has internal symmetry
A palindrome sequence reads the same in both directions (5' to 3') and has internal symmetry. The sticky ends and temperature requirements are properties of the enzyme action, not the palindrome nature.
The most commonly used plasmid vector is:
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pBR322
pBR322 is the most commonly used plasmid vector in recombinant DNA technology. Ti plasmid is used specifically for plant transformation, while lambda and M13 are bacteriophage vectors.
Which organism is most commonly used as competent host in plant biotechnology?
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Agrobacterium tumefaciens
Agrobacterium tumefaciens is the most commonly used competent host in plant biotechnology because of its natural ability to transfer genes into plant cells through its Ti plasmid.
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