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Chapter 12 of 15
Syllabus

Biotechnology — Syllabus

Maharashtra Board · Class 12 · Biology

What Biotechnology covers in Maharashtra Board Class 12 Biology: 4 topics, for the 2026-27 session. Topics include Introduction to Biotechnology.

43 questions25 flashcards5 concepts

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4 Topics · Maharashtra Board Class 12 Biology · 2026-27

Topics in Biotechnology

1

Introduction to Biotechnology

  • Biotechnology defined as 'development and utilization of biological forms, products or processes for obtaining maximum benefits to man and other forms of life'
  • Term first used by Karl Ereky in 1919 for large-scale pig production
  • OECD definition: 'Application of scientific and engineering principles to processing of materials by biological agents'
2

Principles and Processes of Biotechnology

  • Modern biotechnology based on two core techniques: genetic engineering and chemical engineering
  • Genetic engineering deals with alteration of genetic material (DNA and RNA)
  • Chemical engineering maintains sterile environment for manufacturing products
3

Tools and Techniques for Gene Cloning

  • Three categories of tools: Instruments (devices), Biological tools, and Techniques
  • Instruments include electrophoresis, PCR, spectroscopy, chromatography
  • Biological tools: enzymes, cloning vectors, competent hosts
4

Biological Tools - Enzymes

  • Restriction enzymes are molecular scissors that cut DNA at specific sequences
  • Also called restriction endonucleases (REN) or restriction enzymes (RE)
  • Serve as bacterial defense mechanism against viral DNA

Key Concepts

Traditional biotechnology relied on fermentation processesA sophisticated technique involving the manipulationAn automated technique discovered by KaryEssential components include restriction enzymes (molecularLiving organisms with artificially manipulated genetic

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Frequently Asked Questions

What are the important topics in Biotechnology for Maharashtra Board Class 12 Biology?
Key topics in Biotechnology include Introduction to Biotechnology, Principles and Processes of Biotechnology, Tools and Techniques for Gene Cloning, Biological Tools - Enzymes. Study these first, then practise questions on each for the Maharashtra Board Class 12 board exam.
How should I revise Biotechnology for the Maharashtra Board Class 12 board exam?
Learn the core ideas first, then work through the 43 practice questions on Biotechnology. Revise definitions regularly and use flashcards for quick recall before the exam.

Sources & Official References

Content is aligned to the official syllabus. Refer to the board website for the latest curriculum.

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