Plant Tissue Culture — Important Questions
Telangana Open School (TOSS) · Class 12 · Biology
48 important questions from Plant Tissue Culture for Telangana Open School (TOSS) Class 12 Biology, with answers. Written for the board exams 2027.
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Important Questions from Plant Tissue Culture
What is the pH range for the nutrient medium in plant tissue culture?
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5.6 to 6.0
The pH of the nutrient medium is adjusted to 5.6–6.0 to support optimal growth of plant tissues.
Which substance is added to solidify the nutrient medium?
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Agar
Agar is added to the liquid nutrient medium to solidify it and provide support for explants.
What is the purpose of autoclaving the culture medium?
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To kill microorganisms
Autoclaving at 121°C and 15 psi for 15 minutes sterilizes the medium by killing all microorganisms.
At what temperature is the culture medium autoclaved?
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121°C
The standard autoclaving temperature is 121°C at 15 pounds per square inch pressure for 15 minutes.
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