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Plant Tissue Culture

Telangana Open School (TOSS) · Class 12 · Biology

Complete topic list for Plant Tissue Culture in Telangana Open School (TOSS) Class 12 Biology. Key concepts, sub-topics, and what to focus on for board exams.

48 questions30 flashcards5 concepts

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4 Topics · Telangana Open School (TOSS) Class 12 Biology

Topics in Plant Tissue Culture

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1. Totipotency

  • Totipotency refers to the ability of a single plant cell to divide and differentiate into a complete plant under suitable conditions.
  • The term was first coined by Morgan in 1901 to describe regenerative capacity.
  • This concept is the foundation of plant tissue culture because any living plant cell can potentially regenerate into a full plant.
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2. Steps in Plant Tissue Culture

  • The process involves six main steps: preparation of nutrient medium, sterilization, explant preparation, inoculation, incubation, and acclimatization.
  • Each step must be performed under sterile conditions to prevent contamination.
  • The success of tissue culture depends on precise control of nutrients, hormones, temperature, and light.
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3. Nutrient Medium and Growth Regulators

  • The nutrient medium provides all essential elements for plant growth in vitro.
  • MS medium (Murashige and Skoog) is the most commonly used medium.
  • It contains macro and micronutrients, vitamins, amino acids, and carbohydrates like sucrose.
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4. Organogenesis and Somatic Embryogenesis

  • Organogenesis is the formation of organs (roots or shoots) from callus.
  • High auxin : low cytokinin ratio promotes root formation (rhizogenesis).
  • Low auxin : high cytokinin ratio promotes shoot formation (caulogenesis).

Key Concepts

Central concept: Plant Tissue Culture

Cellular TotipotencyNutrient Medium PreparationSterilization of MediumExplantInoculationIncubation for GrowthAcclimatization and TransferApplications of Tissue Culture

Frequently Asked Questions

What are the important topics in Plant Tissue Culture for Telangana Open School (TOSS) Class 12 Biology?
Plant Tissue Culture covers several key topics that are frequently asked in Telangana Open School (TOSS) Class 12 board exams. Focus on the core concepts listed on this page and practise related questions to build confidence.
How to score full marks in Plant Tissue Culture — Telangana Open School (TOSS) Class 12 Biology?
Understand the core concepts first, then work through the 48 practice questions available for this chapter. Revise formulas and definitions regularly, and use flashcards for quick recall before the exam.

Sources & Official References

Content is aligned to the official syllabus. Refer to the board website for the latest curriculum.

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