Skip to main content
Super TutorResources
Chapter 38 of 41
Syllabus

Plant Tissue Culture — Syllabus

Telangana Open School (TOSS) · Class 12 · Biology

What Plant Tissue Culture covers in Telangana Open School (TOSS) Class 12 Biology: 4 topics, for the 2026-27 session.

48 questions30 flashcards5 concepts

Interactive on Super Tutor

Studying Plant Tissue Culture? Get the full interactive chapter.

Quizzes, flashcards, AI doubt-solver and a step-by-step study plan — built for syllabus and more.

Try Super Tutor — Free

Free trial, no card needed.

4 Topics · Telangana Open School (TOSS) Class 12 Biology · 2026-27

Topics in Plant Tissue Culture

1

1. Totipotency

  • Totipotency refers to the ability of a single plant cell to divide and differentiate into a complete plant under suitable conditions.
  • The term was first coined by Morgan in 1901 to describe regenerative capacity.
  • This concept is the foundation of plant tissue culture because any living plant cell can potentially regenerate into a full plant.
2

2. Steps in Plant Tissue Culture

  • The process involves six main steps: preparation of nutrient medium, sterilization, explant preparation, inoculation, incubation, and acclimatization.
  • Each step must be performed under sterile conditions to prevent contamination.
  • The success of tissue culture depends on precise control of nutrients, hormones, temperature, and light.
3

3. Nutrient Medium and Growth Regulators

  • The nutrient medium provides all essential elements for plant growth in vitro.
  • MS medium (Murashige and Skoog) is the most commonly used medium.
  • It contains macro and micronutrients, vitamins, amino acids, and carbohydrates like sucrose.
4

4. Organogenesis and Somatic Embryogenesis

  • Organogenesis is the formation of organs (roots or shoots) from callus.
  • High auxin : low cytokinin ratio promotes root formation (rhizogenesis).
  • Low auxin : high cytokinin ratio promotes shoot formation (caulogenesis).

Key Concepts

Central concept: Plant Tissue Culture

Cellular TotipotencyNutrient Medium PreparationSterilization of MediumExplantInoculationIncubation for GrowthAcclimatization and TransferApplications of Tissue Culture

Work through every topic in Plant Tissue Culture with notes and quizzes

Start This Chapter

Frequently Asked Questions

What are the important topics in Plant Tissue Culture for Telangana Open School (TOSS) Class 12 Biology?
Key topics in Plant Tissue Culture include Totipotency, Steps in Plant Tissue Culture, Nutrient Medium and Growth Regulators, Organogenesis and Somatic Embryogenesis. Study these first, then practise questions on each for the Telangana Open School (TOSS) Class 12 board exam.
How should I revise Plant Tissue Culture for the Telangana Open School (TOSS) Class 12 board exam?
Learn the core ideas first, then work through the 48 practice questions on Plant Tissue Culture. Revise definitions regularly and use flashcards for quick recall before the exam.

Sources & Official References

Content is aligned to the official syllabus. Refer to the board website for the latest curriculum.

More resources for Plant Tissue Culture

All Telangana Open School (TOSS) Class 12 Biology Syllabus

For serious students

Get the full Plant Tissue Culture chapter — start free.

Quizzes, flashcards, an AI doubt solver and a study plan for Telangana Open School (TOSS) Class 12 Biology. Free to start, no card needed.

Try Super Tutor Free
Try this chapter free