Applications of Biotechnology
Tamil Nadu Board · Class 12 · Zoology
Quick revision notes for Applications of Biotechnology — Tamil Nadu Board Class 12 Zoology. Key concepts, formulas, and definitions for last-minute revision.
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10.1 Applications in Medicine - Recombinant Insulin Production
- Insulin is a 51-amino acid hormone produced by β cells of Islets of Langerhans in pancreas
- Insulin consists of two polypeptide chains: Chain A (21 amino acids) and Chain B (30 amino acids) linked by disulfide bonds
- Normal insulin from pig and cow pancreas caused allergic reactions due to structural differences
10.1.2 Human Alpha Lactalbumin and Transgenic Animals
- Alpha lactalbumin is a 123-amino acid protein with 4 disulfide bridges and molecular weight of 14178 Da
- Most abundant protein in human milk (25% of total protein), synthesized by mammary glands
- Binds calcium and zinc ions, possesses bactericidal and anti-tumor activities
10.1.3 Interferons - Antiviral Proteins
- Interferons are proteinaceous, antiviral, species-specific substances produced by mammalian cells infected with viruses
- Discovered by Alick Isaacs and Jean Lindemann in 1957
- Classified as α (alpha), β (beta), and γ (gamma) interferons based on structure
10.1.4 Recombinant Vaccines and Vaccine Technology
- Recombinant vaccines overcome limitations of traditional vaccine production methods
- Uniform quality with less side effects compared to conventional vaccines
- Three main types: subunit recombinant vaccines, attenuated recombinant vaccines, and DNA vaccines
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