Tools and Techniques
CBSE · Class 11 · Biotechnology
Summary of Tools and Techniques for CBSE Class 11 Biotechnology. Key concepts, important points, and chapter overview.
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Overview
Tools and techniques in biotechnology provide the practical methods needed to observe cells, separate biomolecules, measure reactions, identify genes, sequence DNA, and study cells in large numbers. Microscopy reveals structures too small for the naked eye, centrifugation separates particles by dens
Key Concepts
Microscopy increases the visible size
Microscopy increases the visible size of very small objects. Bright field microscopy uses light for illumination and usually needs stains. Dark field
Magnification is the increase in retinal
Magnification is the increase in retinal image size. Total magnification of a compound microscope equals objective magnification multiplied by eyepiec
Centrifugation separates particles under the influence
Centrifugation separates particles under the influence of centrifugal force. Differential centrifugation separates particles with different size and d
Electrophoresis separates charged molecules under
Electrophoresis separates charged molecules under an electric field. DNA, RNA, peptides, and proteins move according to charge and size. Mobility is i
Bromophenol blue is a common tracking
Bromophenol blue is a common tracking dye, coloured at alkaline and neutral pH, and highly mobile. Xylene cyanol has lower mobility, while Orange G ha
Learning Objectives
- Understand the major laboratory tools used in biotechnology
- Distinguish between different forms of microscopy and their uses
- Explain how centrifugation separates cellular components
- Describe the principle of electrophoresis and its main types
- Compare the four types of ELISA
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Sources & Official References
- NCERT Official — ncert.nic.in
- CBSE Academic — cbseacademic.nic.in
- CBSE Official — cbse.gov.in
- National Education Policy 2020 — education.gov.in
Content is aligned to the official syllabus. Refer to the board website for the latest curriculum.
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