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Syllabus

Microbial Culture — Syllabus

CBSE · Class 12 · Biotechnology

What Microbial Culture covers in CBSE Class 12 Biotechnology: 4 topics, for the 2026-27 session. Part of the CBSE Class 12 Biotechnology syllabus.

63 questions84 flashcards5 concepts

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A comparison diagram showing Louis Pasteur's swan-neck flask experiment, illustrating how life does not arise spontaneously from non-living matter.
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4 Topics · CBSE Class 12 Biotechnology · 2026-27

Topics in Microbial Culture

1

Historical Perspective

  • Antonie van Leeuwenhoek developed the microscope in the 1670s to 1680s and observed microscopic organisms, which he called animalcules.
  • Lazzaro Spallanzani found no microscopic life in boiled broth, which challenged spontaneous generation.
  • Louis Pasteur used swan-neck flask experiments and disproved spontaneous generation.
2

Nutritional Requirements and Types of Media

  • Microorganisms need carbon, oxygen, hydrogen, nitrogen, sulfur, phosphorus, potassium, calcium, magnesium, iron, water, and growth factors for growth and multiplication.
  • Carbon, oxygen, hydrogen, nitrogen, sulfur, phosphorus, potassium, calcium, magnesium, and iron are macronutrients and are needed in relatively large amounts.
  • Heterotrophs obtain carbon from organic molecules.
3

Sterilisation Methods

  • Sterilisation means removing or killing all living microorganisms, including bacterial spores.
  • Sterilisation can be achieved by physical methods and chemical methods.
  • Physical methods include heat, radiation, and filtration.
4

Pure Culture Techniques

  • A pure culture consists of entirely one strain of organism.
  • Pure culture is important for studying a specific microbe.
  • Solid media are especially useful for isolating bacteria and for long-term storage.

Key Concepts

Central concept: Microbial culture as the study of microbial nutrition, media, sterilisation, pure culture techniques, growth factors, and growth curve analysis

Historical perspectiveNutritional categories of microbesNutritional requirementsCulture mediaSterilisation methodsPure culture techniquesFactors affecting microbial growthGrowth curve

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Frequently Asked Questions

What are the important topics in Microbial Culture for CBSE Class 12 Biotechnology?
Key topics in Microbial Culture include Historical Perspective, Nutritional Requirements and Types of Media, Sterilisation Methods, Pure Culture Techniques. Study these first, then practise questions on each for the CBSE Class 12 board exam.
How should I revise Microbial Culture for the CBSE Class 12 board exam?
Learn the core ideas first, then work through the 63 practice questions on Microbial Culture. Revise definitions regularly and use flashcards for quick recall before the exam.

Sources & Official References

Content is aligned to the official syllabus. Refer to the board website for the latest curriculum.

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