Applications of Recombinant DNA Technology
CBSE · Class 12 · Biotechnology
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What is the main use of DNA fingerprinting in forensic science and paternity testing?
Who developed DNA fingerprinting in 1984 at the University of Leicester?
What proportion of the human genome is described as non-coding DNA?
Which term is used for variable number tandem repeats in DNA fingerprinting?
Sample Questions
Which size range matches microsatellites in VNTR family classification?
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Repeated sequences of 1 to 9 bp
Microsatellites are VNTRs with repeated sequences of 1 to 9 base pairs.
Which size range matches minisatellites in VNTR family classification?
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Repeated sequences of 10 to 100 bp
Minisatellites are VNTRs with repeated sequences of 10 to 100 base pairs.
What technique is used to increase the sensitivity of DNA fingerprinting by amplifying DNA?
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PCR
PCR is used to amplify DNA, which increases the sensitivity of DNA fingerprinting even when the sample amount is small.
Which technique is used to detect VNTR bands in DNA fingerprinting?
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Southern hybridisation using VNTR probes
Direct identification of bands is difficult, so Southern hybridisation using VNTR probes is used to detect DNA fragments.
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Sources & Official References
- NCERT Official — ncert.nic.in
- CBSE Academic — cbseacademic.nic.in
- CBSE Official — cbse.gov.in
- National Education Policy 2020 — education.gov.in
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