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Applications of Recombinant DNA Technology — Syllabus

CBSE · Class 12 · Biotechnology

What Applications of Recombinant DNA Technology covers in CBSE Class 12 Biotechnology: 4 topics, for the 2026-27 session.

79 questions106 flashcards5 concepts

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A flowchart illustrating the ex vivo gene therapy approach for treating Severe Combined Immunodeficiency (SCID) caused by Adenosine Deaminase (ADA) deficiency, involving lymphocyte modification and re
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4 Topics · CBSE Class 12 Biotechnology · 2026-27

Topics in Applications of Recombinant DNA Technology

1

DNA Fingerprinting

  • DNA fingerprinting identifies individuals by using inherited variations in DNA.
  • The human genome consists of 3.2 × 10^6 bp.
  • About 99.9% of the human genome is the same in all humans, and the remaining 0.1% contains inherited variations.
2

Transgenic Organisms

  • Transgenic organisms carry a foreign gene introduced by transgenesis.
  • The first genetically modified organism was a bacterium made by Herbert Boyer and Stanley Cohen in 1973.
  • The first transgenic animal, transgenic mice, was engineered by Rudolf Jaenisch and Beatrice Mintz in 1974.
3

Gene Therapy

  • Gene therapy is designed to repair faulty genes by introducing correct genetic material into cells.
  • The first gene therapy was performed in 1990 on a four-year-old girl with SCID caused by ADA gene defect.
  • In this case, a functional ADA gene was inserted into a viral vector and introduced into lymphocytes.
4

Recombinant Vaccines

  • A vaccine is a preparation of killed or weakened pathogen or its components that induces immunity.
  • Edward Jenner coined the word vaccine from vacca, meaning cow, and used cowpox virus to protect against smallpox.
  • Louis Pasteur coined the term vaccination in 1881.

Key Concepts

Central concept: Applications of recombinant DNA technology in medicine, agriculture, diagnostics, and biological research

DNA fingerprintingTransgenic organismsTransgenic plantsTransgenic animalsGene therapyRecombinant vaccinesMonoclonal antibodiesInsulin production

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Frequently Asked Questions

What are the important topics in Applications of Recombinant DNA Technology for CBSE Class 12 Biotechnology?
Key topics in Applications of Recombinant DNA Technology include DNA Fingerprinting, Transgenic Organisms, Gene Therapy, Recombinant Vaccines. Study these first, then practise questions on each for the CBSE Class 12 board exam.
How should I revise Applications of Recombinant DNA Technology for the CBSE Class 12 board exam?
Learn the core ideas first, then work through the 79 practice questions on Applications of Recombinant DNA Technology. Revise definitions regularly and use flashcards for quick recall before the exam.

Sources & Official References

Content is aligned to the official syllabus. Refer to the board website for the latest curriculum.

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