Gene Cloning
CBSE · Class 12 · Biotechnology
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What is gene cloning in biotechnology best described as?
What is the first and most difficult problem in gene cloning?
Which DNA size is used as an example to show the difficulty of finding a gene of interest in the human genome?
What is the approximate size of a haploid human genome?
Sample Questions
What is the role of reverse transcriptase in cDNA preparation?
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It makes cDNA from an RNA template
Reverse transcriptase is an RNA-directed DNA polymerase that synthesises cDNA using RNA as the template.
Which molecule is used to break the peptidoglycan of bacterial cell walls during DNA isolation?
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Lysozyme
Lysozyme digests peptidoglycan, the main component of the bacterial cell wall.
Which detergent is used for bacterial cell lysis by disrupting the lipid bilayer?
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SDS
SDS is used to lyse cell membranes by disrupting the lipid bilayer.
What type of detergent is CTAB?
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Cationic detergent
CTAB is used for plant DNA isolation and is a cationic detergent.
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Sources & Official References
- NCERT Official — ncert.nic.in
- CBSE Academic — cbseacademic.nic.in
- CBSE Official — cbse.gov.in
- National Education Policy 2020 — education.gov.in
Content is aligned to the official syllabus. Refer to the board website for the latest curriculum.
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