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Applications of Recombinant DNA Technology

CBSE · Class 12 · Biotechnology

Quick revision notes for Applications of Recombinant DNA Technology — CBSE Class 12 Biotechnology. Key concepts, formulas, and definitions for last-minute revision.

80 questions106 flashcards5 concepts

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A flowchart illustrating the ex vivo gene therapy approach for treating Severe Combined Immunodeficiency (SCID) caused by Adenosine Deaminase (ADA) deficiency, involving lymphocyte modification and re
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Key Topics to Revise

1

DNA Fingerprinting

  • DNA fingerprinting identifies individuals by using inherited variations in DNA.
  • The human genome consists of 3.2 × 10^6 bp.
  • About 99.9% of the human genome is the same in all humans, and the remaining 0.1% contains inherited variations.
2

Transgenic Organisms

  • Transgenic organisms carry a foreign gene introduced by transgenesis.
  • The first genetically modified organism was a bacterium made by Herbert Boyer and Stanley Cohen in 1973.
  • The first transgenic animal, transgenic mice, was engineered by Rudolf Jaenisch and Beatrice Mintz in 1974.
3

Gene Therapy

  • Gene therapy is designed to repair faulty genes by introducing correct genetic material into cells.
  • The first gene therapy was performed in 1990 on a four-year-old girl with SCID caused by ADA gene defect.
  • In this case, a functional ADA gene was inserted into a viral vector and introduced into lymphocytes.
4

Recombinant Vaccines

  • A vaccine is a preparation of killed or weakened pathogen or its components that induces immunity.
  • Edward Jenner coined the word vaccine from vacca, meaning cow, and used cowpox virus to protect against smallpox.
  • Louis Pasteur coined the term vaccination in 1881.

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Full Notes

Key Concepts

DNA fingerprinting compares inherited DNA variationsMore than 90% of the humanTransgenic organisms carry a foreign geneTransgenic plants are created by vectorCommon methods include DNA pronuclear microinjection

Frequently Asked Questions

What are the important topics in Applications of Recombinant DNA Technology for CBSE Class 12 Biotechnology?
Key topics in Applications of Recombinant DNA Technology include Overview of Applications of Recombinant DNA Technology, Applications of Recombinant DNA Technology — Complete Chapter Overview, Chapter 4: Applications of rDNA Technology — Complete Overview. These are the concepts CBSE Class 12 examiners draw on most — study them first, then practise related questions.
How to score full marks in Applications of Recombinant DNA Technology — CBSE Class 12 Biotechnology?
Understand the core concepts first, then work through the 80 practice questions available for this chapter. Revise formulas and definitions regularly, and use flashcards for quick recall before the exam.

Sources & Official References

Content is aligned to the official syllabus. Refer to the board website for the latest curriculum.

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