Applications of Recombinant DNA Technology
CBSE · Class 12 · Biotechnology
Summary of Applications of Recombinant DNA Technology for CBSE Class 12 Biotechnology. Key concepts, important points, and chapter overview.
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Recombinant DNA (rDNA) technology has transformed modern science and medicine by enabling scientists to manipulate genetic material in ways that were once unimaginable. This chapter explores four major application areas: DNA fingerprinting (identifying individuals based on unique DNA patterns), tran
Key Concepts
Developed by Sir Alec Jeffreys
Developed by Sir Alec Jeffreys in 1984, DNA fingerprinting identifies individuals based on unique variations in their DNA sequence. About 99.9% of hum
Transgenesis involves inserting a foreign gene
Transgenesis involves inserting a foreign gene (transgene) into an organism's genome so that it is transmitted and expressed in offspring. The resulti
Agrobacterium tumefaciens is a natural genetic
Agrobacterium tumefaciens is a natural genetic engineer that causes crown gall disease by transferring its T-DNA (tumor-inducing DNA) from its Ti plas
Antisense technology silences gene expression
Antisense technology silences gene expression. In antisense oligonucleotide (ASO) technique, a synthetic oligonucleotide complementary to target mRNA
Gene therapy repairs or replaces faulty
Gene therapy repairs or replaces faulty genes by introducing correct genetic material into cells. The first gene therapy was performed in 1990 on a fo
Learning Objectives
- Understand the principle and procedure of DNA fingerprinting using VNTR and RFLP techniques
- Explain the methods used to produce transgenic plants and animals
- Describe the applications and concerns associated with genetically modified organisms (GMOs)
- Understand the concept, approaches, types, and ethical issues of gene therapy
- Differentiate between types of recombinant vaccines and their mechanisms
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Sources & Official References
- NCERT Official — ncert.nic.in
- CBSE Academic — cbseacademic.nic.in
- CBSE Official — cbse.gov.in
- National Education Policy 2020 — education.gov.in
Content is aligned to the official syllabus. Refer to the board website for the latest curriculum.
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