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Gene Cloning

CBSE · Class 12 · Biotechnology

Flashcards for Gene Cloning — CBSE Class 12 Biotechnology. Quick Q&A cards covering key concepts, definitions, and formulas.

72 questions88 flashcards5 concepts

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A diagram illustrating the concept of insertional inactivation using a chromogenic substrate, where insertion of foreign DNA into a gene (e.g., β-galactosidase) inactivates it, leading to a visible ch
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88 Flashcards
Card 1जीन क्लोनिंग का परिचय

जीन क्लोनिंग क्या है?

Answer

जीन क्लोनिंग जैव प्रौद्योगिकी उद्योग और अन्य विभिन्न उद्देश्यों के लिए एक जीन का उपयोग करने के लिए एक प्रथागत प्रक्रिया है। इसमें रुचि के जीन वाले DNA खंड को एक वेक्टर द्वारा एक मेजबान कोशिका में स्था

Card 2Introduction to gene cloning

What is gene cloning?

Answer

Gene cloning is a customary procedure to use a gene for its product in the biotechnology industry and various other purposes. It involves transferring a DNA fragment containing the gene of interest in

Card 3Identification of candidate gene

Why is finding a candidate gene difficult in a genome?

Answer

A genome is very large, so a target gene is only a tiny part of it. A haploid human genome contains approximately 3.2 billion bp, and a gene of interest may be only 3000 to 3500 bp. That makes gene id

Card 4उम्मीदवार जीन की पहचान

जीनोम में एक उम्मीदवार जीन खोजना मुश्किल क्यों है?

Answer

एक जीनोम बहुत बड़ा होता है, इसलिए एक लक्षित जीन इसका एक छोटा सा हिस्सा होता है। एक हैप्लोइड मानव जीनोम में लगभग 3.2 बिलियन बीपी होते हैं, और रुचि का एक जीन केवल 3000 से 3500 बीपी का हो सकता है। यह जीन

Card 5उम्मीदवार जीन विधियाँ

क्लोनिंग के लिए उम्मीदवार जीन प्राप्त करने के दो तरीके क्या हैं?

Answer

एक तरीका है अमीनो एसिड अनुक्रम से जीन के DNA अनुक्रम का अनुमान लगाना। दूसरा तरीका है कि विशिष्ट ऊतक से mRNA को अलग किया जाए, रिवर्स ट्रांसक्रिप्टेस का उपयोग करके एकल-स्ट्रैंडेड cDNA बनाया जाए और फिर क

Card 6Candidate gene methods

What are two ways to obtain a candidate gene for cloning?

Answer

One way is to deduce the DNA sequence of the gene from its amino acid sequence. Another way is to isolate mRNA from the specific tissue, make single-stranded cDNA using reverse transcriptase, and then

Card 7Isolation of nucleic acids

Why is nucleic acid isolation necessary in gene cloning?

Answer

Nucleic acid isolation is the first essential step in molecular biology work. DNA and RNA are present in very small amounts in cells and are easily damaged by physical stress and chemical agents, so t

Card 8न्यूक्लिक अम्लों का पृथक्करण

जीन क्लोनिंग में न्यूक्लिक एसिड पृथक्करण क्यों आवश्यक है?

Answer

आणविक जीवविज्ञान के कार्य में न्यूक्लिक अम्ल का पृथक्करण पहला आवश्यक चरण है। DNA और RNA कोशिकों में बहुत कम मात्रा में उपस्थित होते हैं और भौतिक तनाव तथा रासायनिक कारकों से आसानी से क्षतिग्रस्त हो जात

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What are the important topics in Gene Cloning for CBSE Class 12 Biotechnology?
Key topics in Gene Cloning include Gene Cloning — Complete Chapter Overview, Gene Cloning — Chapter Overview, Gene Cloning — Complete Chapter Overview. These are the concepts CBSE Class 12 examiners draw on most — study them first, then practise related questions.
How to score full marks in Gene Cloning — CBSE Class 12 Biotechnology?
Understand the core concepts first, then work through the 72 practice questions available for this chapter. Revise formulas and definitions regularly, and use flashcards for quick recall before the exam.
How many flashcards are available for Gene Cloning?
There are 88 flashcards for Gene Cloning covering key definitions, formulas, and concepts. Use them daily for 10–15 minutes for best results.

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