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Host–Vector System

CBSE · Class 12 · Biotechnology

Step-by-step guide to study Host–Vector System in CBSE Class 12 Biotechnology. Topics to cover, practice strategy, and time allocation.

64 questions80 flashcards5 concepts

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A flowchart illustrating the two major steps of recombinant DNA technology: isolation of target gene (insert) and its insertion into a vector to form recombinant DNA (rDNA), followed by introduction i
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Study Plan

1
Day 1–2

Learn the Theory

Read the textbook chapter carefully. Note down definitions, formulas, and key concepts.

2
Day 3

Practice Problems

Solve textbook exercises and additional practice questions. There are 64 questions available for this chapter.

3
Day 4

Revise & Test

Revise key formulas and concepts without looking at notes. Take a practice quiz to test your understanding. Mark weak areas for re-revision.

4
Day 7

Spaced Revision

Revisit Host–Vector System after a week. Use flashcards for quick recall. Solve previous year questions from this chapter.

What to Focus On

  • Recombinant DNA technology uses both a host and a vector.
  • The insert is the target DNA; the vector carries it.
  • The host provides replication functions.

  • E. coli is the most extensively used prokaryotic host.
  • E. coli K12 is commonly used in gene cloning.
  • Bacillus subtilis is useful when protein secretion is the goal.

  • Plasmids are circular, ds, extra-chromosomal DNA.
  • Episomes are plasmids that can integrate into the host chromosome.
  • R-plasmids confer antibiotic resistance.

Common Mistakes to Avoid

A vector alone can do the cloning work without a host.

Any DNA molecule can be used as a vector if it can enter a cell.

E. coli is used as a host only because it is common, not because it grows rapidly.

Memory Tips

Core idea of recombinant DNA technology as a host-vector system

E. coli as the most extensively used prokaryotic host

K12 strain of E. coli is commonly used in gene cloning

Bacillus subtilis as an alternative prokaryotic host for secretion

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Frequently Asked Questions

What are the important topics in Host–Vector System for CBSE Class 12 Biotechnology?
Host–Vector System covers several key topics that are frequently asked in CBSE Class 12 board exams. Focus on the core concepts listed on this page and practise related questions to build confidence.
How to score full marks in Host–Vector System — CBSE Class 12 Biotechnology?
Understand the core concepts first, then work through the 64 practice questions available for this chapter. Revise formulas and definitions regularly, and use flashcards for quick recall before the exam.

Sources & Official References

Content is aligned to the official syllabus. Refer to the board website for the latest curriculum.

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