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Host–Vector System — Revision Notes

CBSE · Class 12 · Biotechnology

Host–Vector System revision notes for CBSE Class 12 Biotechnology: 4 topics in quick points. Part of the CBSE Class 12 Biotechnology syllabus.

64 questions80 flashcards5 concepts

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A flowchart illustrating the two major steps of recombinant DNA technology: isolation of target gene (insert) and its insertion into a vector to form recombinant DNA (rDNA), followed by introduction i
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Key Topics to Revise

1

1. Core idea of host–vector system

  • Recombinant DNA technology works through a compatible host and a vector.
  • The desired DNA molecule is called the insert or target gene.
  • The carrier DNA molecule is called the vector.
2

2. Features of a good vector

  • A vector should be small in size.
  • A vector should have an origin of replication, or ori.
  • A vector should have unique restriction sites.
3

3. Plasmids as vectors

  • Plasmids are circular, double-stranded, extra chromosomal DNA capable of autonomous replication.
  • Plasmids occur naturally in bacteria, archaea, and yeast.
  • Plasmids can integrate into chromosomes; such plasmids are called episomes.
4

4. Development of plasmid vectors

  • pBR313 was an early plasmid-based vector and was 9 kb in size.
  • pBR313 carried tetR and ampR selectable markers.
  • pBR313 replicated in a relaxed fashion.

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Full Notes

Key Concepts

A host is the organism intoA vector is a DNA moleculePlasmids are circularpBR313 was an original plasmid vectorpUC19 is an improved plasmid cloning

Frequently Asked Questions

What are the important topics in Host–Vector System for CBSE Class 12 Biotechnology?
Key topics in Host–Vector System include Core idea of host–vector system, Features of a good vector, Plasmids as vectors, Development of plasmid vectors. Study these first, then practise questions on each for the CBSE Class 12 board exam.
How should I revise Host–Vector System for the CBSE Class 12 board exam?
Learn the core ideas first, then work through the 64 practice questions on Host–Vector System. Revise definitions regularly and use flashcards for quick recall before the exam.

Sources & Official References

Content is aligned to the official syllabus. Refer to the board website for the latest curriculum.

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All CBSE Class 12 Biotechnology Revision Notes

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