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Revision Notes

Host–Vector System

CBSE · Class 12 · Biotechnology

Quick revision notes for Host–Vector System — CBSE Class 12 Biotechnology. Key concepts, formulas, and definitions for last-minute revision.

64 questions80 flashcards5 concepts

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A flowchart illustrating the two major steps of recombinant DNA technology: isolation of target gene (insert) and its insertion into a vector to form recombinant DNA (rDNA), followed by introduction i
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Key Topics to Revise

1

1. Core idea of host–vector system

  • Recombinant DNA technology works through a compatible host and a vector.
  • The desired DNA molecule is called the insert or target gene.
  • The carrier DNA molecule is called the vector.
2

2. Features of a good vector

  • A vector should be small in size.
  • A vector should have an origin of replication, or ori.
  • A vector should have unique restriction sites.
3

3. Plasmids as vectors

  • Plasmids are circular, double-stranded, extra chromosomal DNA capable of autonomous replication.
  • Plasmids occur naturally in bacteria, archaea, and yeast.
  • Plasmids can integrate into chromosomes; such plasmids are called episomes.
4

4. Development of plasmid vectors

  • pBR313 was an early plasmid-based vector and was 9 kb in size.
  • pBR313 carried tetR and ampR selectable markers.
  • pBR313 replicated in a relaxed fashion.

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Full Notes

Key Concepts

A host is the organism intoA vector is a DNA moleculePlasmids are circularpBR313 was an original plasmid vectorpUC19 is an improved plasmid cloning

Frequently Asked Questions

What are the important topics in Host–Vector System for CBSE Class 12 Biotechnology?
Host–Vector System covers several key topics that are frequently asked in CBSE Class 12 board exams. Focus on the core concepts listed on this page and practise related questions to build confidence.
How to score full marks in Host–Vector System — CBSE Class 12 Biotechnology?
Understand the core concepts first, then work through the 64 practice questions available for this chapter. Revise formulas and definitions regularly, and use flashcards for quick recall before the exam.

Sources & Official References

Content is aligned to the official syllabus. Refer to the board website for the latest curriculum.

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