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Chapter Summary

Host–Vector System

CBSE · Class 12 · Biotechnology

Summary of Host–Vector System for CBSE Class 12 Biotechnology. Key concepts, important points, and chapter overview.

64 questions80 flashcards5 concepts

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A flowchart illustrating the two major steps of recombinant DNA technology: isolation of target gene (insert) and its insertion into a vector to form recombinant DNA (rDNA), followed by introduction i
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Overview

The host–vector system is the basic framework of recombinant DNA technology. A vector carries the desired DNA fragment, called the insert, into a compatible host. The host then provides the cellular machinery needed for replication and expression of the recombinant DNA. Together, the host and vector

Key Concepts

A host is the organism into

A host is the organism into which recombinant DNA is introduced. It supports replication and expression of the inserted gene. E. coli is the most exte

A vector is a DNA molecule

A vector is a DNA molecule capable of self-replication inside a host cell. It must be small, carry the insert, contain an origin of replication, have

Plasmids are circular

Plasmids are circular, double-stranded, extra-chromosomal DNA molecules capable of autonomous replication. Some integrate into chromosomes and are cal

pBR313 was an original plasmid vector

pBR313 was an original plasmid vector of 9 kb with tetR and ampR markers and several unique restriction sites. pBR322 was developed by deleting most n

pUC19 is an improved plasmid cloning

pUC19 is an improved plasmid cloning vector with ori, ampR, and a multiple cloning site. It also contains part of the lacZ gene, which allows blue/whi

Learning Objectives

  • Understand the role of host and vector in recombinant DNA technology
  • Identify the qualities of a good cloning vector
  • Describe major prokaryotic and eukaryotic hosts used in gene cloning
  • Differentiate between plasmid, bacteriophage, cosmid, phasmid, and artificial chromosome vectors
  • Explain the structural and functional features of important vectors such as pBR322, pUC19, λ phage vectors, M13 vectors, YAC, BAC, and PAC

Frequently Asked Questions

What are the important topics in Host–Vector System for CBSE Class 12 Biotechnology?
Host–Vector System covers several key topics that are frequently asked in CBSE Class 12 board exams. Focus on the core concepts listed on this page and practise related questions to build confidence.
How to score full marks in Host–Vector System — CBSE Class 12 Biotechnology?
Understand the core concepts first, then work through the 64 practice questions available for this chapter. Revise formulas and definitions regularly, and use flashcards for quick recall before the exam.

Sources & Official References

Content is aligned to the official syllabus. Refer to the board website for the latest curriculum.

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