Plant Tissue Culture
CBSE · Class 12 · Biotechnology
Summary of Plant Tissue Culture for CBSE Class 12 Biotechnology. Key concepts, important points, and chapter overview.
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Plant tissue culture is the cultivation of undifferentiated plant cells, tissues, or organs on synthetic media under aseptic conditions and controlled physical conditions. Its foundation is totipotency, the ability of a vegetative cell to divide, differentiate into specialised cells, or regenerate i
Key Concepts
Plant tissue culture is the growth
Plant tissue culture is the growth of plant cells, tissues, or organs on artificial nutrient media under sterile and controlled conditions. It depends
Totipotency is the ability of
Totipotency is the ability of a vegetative cell to divide and differentiate into any type of specialised cell or to regenerate into a whole plant.
Key milestones include Haberlandt’s early principles
Key milestones include Haberlandt’s early principles, discovery of IAA, White’s synthetic medium, Van Overbeek’s coconut water, discovery of kinetin,
An explant is any plant part
An explant is any plant part used to start culture, such as a leaf, meristem, cotyledon, hypocotyl, or embryo. Surface sterilisation is done to remove
Culture media contain inorganic nutrients
Culture media contain inorganic nutrients, organic supplements, a carbon source, plant growth hormones, gelling agents, and antibiotics. Macronutrient
Learning Objectives
- Understand the meaning of plant tissue culture and totipotency
- Recall the major historical milestones in the development of plant tissue culture
- Identify the main components of nutrient media and their roles
- Differentiate among organ culture, callus culture, cell suspension culture, and protoplast culture
- Explain the steps involved in plant tissue culture from explant selection to plantlet transfer
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Sources & Official References
- NCERT Official — ncert.nic.in
- CBSE Academic — cbseacademic.nic.in
- CBSE Official — cbse.gov.in
- National Education Policy 2020 — education.gov.in
Content is aligned to the official syllabus. Refer to the board website for the latest curriculum.
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