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Plant Tissue Culture — Revision Notes

CBSE · Class 12 · Biotechnology

Plant Tissue Culture revision notes for CBSE Class 12 Biotechnology: 4 topics in quick points. Part of the CBSE Class 12 Biotechnology syllabus.

83 questions72 flashcards5 concepts

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A diagram illustrating the core concept of plant tissue culture: taking a small plant part (explant) and culturing it on synthetic media under aseptic conditions to regenerate a whole plant, highlight
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Key Topics to Revise

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1. Basic idea and historical development

  • Plant tissue culture is the cultivation of undifferentiated plant cells, tissues, or organs on artificial media under aseptic and controlled conditions.
  • Totipotency is the basis of plant tissue culture.
  • Schwann and Schleiden established that the cell is the basic unit of life capable of dividing and growing.
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2. Explants, sterilisation, and basic steps of culture

  • Virtually any plant part such as leaf, apical meristem, embryo, cotyledon, or hypocotyl can be used as an explant.
  • Immature embryos are generally more responsive than apical meristems, and apical meristems are generally more responsive than leaf explants under suitable conditions.
  • Explants from seedlings grown in sterile conditions do not need re-sterilisation.
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3. Nutrient media and its components

  • Success of in vitro culture depends on the right composition of the culture medium.
  • Culture media contain inorganic components, organic supplements, carbon source, plant growth hormones, gelling agents, and antibiotics.
  • Macronutrients are required in milli molar concentrations and include carbon, calcium, hydrogen, potassium, magnesium, nitrogen, oxygen, phosphorus, and sulphur.
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4. Culture types and morphogenesis

  • Plant tissue culture can be categorised as organ culture, callus culture, cell suspension culture, and protoplast culture.
  • Organ culture involves organs such as root, anther, ovary, embryo, endosperm, or seed under laboratory conditions.
  • Callus culture produces an unorganised mass of cells called callus.

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Full Notes

Key Concepts

Plant tissue culture is the growthTotipotency is the ability ofKey milestones include Haberlandt’s early principlesAn explant is any plant partCulture media contain inorganic nutrients

Frequently Asked Questions

What are the important topics in Plant Tissue Culture for CBSE Class 12 Biotechnology?
Key topics in Plant Tissue Culture include Basic idea and historical development, Explants, sterilisation, and basic steps of culture, Nutrient media and its components, Culture types and morphogenesis. Study these first, then practise questions on each for the CBSE Class 12 board exam.
How should I revise Plant Tissue Culture for the CBSE Class 12 board exam?
Learn the core ideas first, then work through the 83 practice questions on Plant Tissue Culture. Revise definitions regularly and use flashcards for quick recall before the exam.

Sources & Official References

Content is aligned to the official syllabus. Refer to the board website for the latest curriculum.

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