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Plant Tissue Culture

CBSE · Class 12 · Biotechnology

Complete topic list for Plant Tissue Culture in CBSE Class 12 Biotechnology. Key concepts, sub-topics, and what to focus on for board exams.

84 questions72 flashcards5 concepts

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A diagram illustrating the core concept of plant tissue culture: taking a small plant part (explant) and culturing it on synthetic media under aseptic conditions to regenerate a whole plant, highlight
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4 Topics · CBSE Class 12 Biotechnology

Topics in Plant Tissue Culture

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1. Basic idea and historical development

  • Plant tissue culture is the cultivation of undifferentiated plant cells, tissues, or organs on artificial media under aseptic and controlled conditions.
  • Totipotency is the basis of plant tissue culture.
  • Schwann and Schleiden established that the cell is the basic unit of life capable of dividing and growing.
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2. Explants, sterilisation, and basic steps of culture

  • Virtually any plant part such as leaf, apical meristem, embryo, cotyledon, or hypocotyl can be used as an explant.
  • Immature embryos are generally more responsive than apical meristems, and apical meristems are generally more responsive than leaf explants under suitable conditions.
  • Explants from seedlings grown in sterile conditions do not need re-sterilisation.
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3. Nutrient media and its components

  • Success of in vitro culture depends on the right composition of the culture medium.
  • Culture media contain inorganic components, organic supplements, carbon source, plant growth hormones, gelling agents, and antibiotics.
  • Macronutrients are required in milli molar concentrations and include carbon, calcium, hydrogen, potassium, magnesium, nitrogen, oxygen, phosphorus, and sulphur.
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4. Culture types and morphogenesis

  • Plant tissue culture can be categorised as organ culture, callus culture, cell suspension culture, and protoplast culture.
  • Organ culture involves organs such as root, anther, ovary, embryo, endosperm, or seed under laboratory conditions.
  • Callus culture produces an unorganised mass of cells called callus.

Key Concepts

Central concept: Plant tissue culture as the cultivation of plant cells, tissues, or organs on synthetic media under aseptic and controlled conditions based on totipotency

TotipotencyHistorical developmentExplant and sterilisationNutrient mediaMacronutrients and micronutrientsPlant growth hormonesCulture typesOrganogenesis

Frequently Asked Questions

What are the important topics in Plant Tissue Culture for CBSE Class 12 Biotechnology?
Plant Tissue Culture covers several key topics that are frequently asked in CBSE Class 12 board exams. Focus on the core concepts listed on this page and practise related questions to build confidence.
How to score full marks in Plant Tissue Culture — CBSE Class 12 Biotechnology?
Understand the core concepts first, then work through the 84 practice questions available for this chapter. Revise formulas and definitions regularly, and use flashcards for quick recall before the exam.

Sources & Official References

Content is aligned to the official syllabus. Refer to the board website for the latest curriculum.

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