Bioprocessing and Biomanufacturing
CBSE · Class 12 · Biotechnology
Complete topic list for Bioprocessing and Biomanufacturing in CBSE Class 12 Biotechnology. Key concepts, sub-topics, and what to focus on for board exams.
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1. Core Ideas and Historical Background
- Metabolites are chemical compounds formed by living organisms and are broadly of two types: primary metabolites and secondary metabolites.
- Primary metabolites are produced directly from primary metabolic pathways and are linked to essential cellular functions such as growth and development.
- Secondary metabolites are produced by different secondary metabolic pathways and often help in defence, stress tolerance, attraction of pollinators or dispersal agents, and protection against unwanted
2. Bioreactor and Fermenter Design
- At the industrial stage, bioprocesses are carried out in vessels called fermenters or bioreactors.
- A bioreactor is an engineered vessel made of glass or steel that supports a biologically active environment under aseptic conditions.
- A bioreactor must provide sterility, adequate air supply, uniform mixing, optimum temperature, and monitoring of pH and dissolved oxygen.
3. Upstream Processing and Operating Conditions
- A bioprocess has two main stages: upstream processing and downstream processing.
- Upstream processing includes medium formulation, sterilisation, inoculum preparation, and optimisation of environmental conditions.
- Culture media are prepared to support maximum growth and product formation.
4. Modes of Bioprocess Operation
- The three modes of bioprocess operation are batch mode, fed-batch mode, and continuous mode.
- Batch mode is a closed culture system with a fixed initial amount of nutrients.
- In batch culture, cells pass through phases of growth and convert substrates into product.
Key Concepts
Central concept: Bioprocessing and Biomanufacturing
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Sources & Official References
- NCERT Official — ncert.nic.in
- CBSE Academic — cbseacademic.nic.in
- CBSE Official — cbse.gov.in
- National Education Policy 2020 — education.gov.in
Content is aligned to the official syllabus. Refer to the board website for the latest curriculum.
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