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Animal Cell Culture

CBSE · Class 12 · Biotechnology

Complete topic list for Animal Cell Culture in CBSE Class 12 Biotechnology. Key concepts, sub-topics, and what to focus on for board exams.

77 questions76 flashcards5 concepts

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An infographic illustrating the basic concept of animal cell culture, showing cells isolated from an animal tissue and then maintained and proliferated in vitro under controlled laboratory conditions.
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4 Topics · CBSE Class 12 Biotechnology

Topics in Animal Cell Culture

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1. Meaning, Features, and Basic Idea of Animal Cell Culture

  • Animal cell culture means keeping animal cells alive and multiplying them outside the body under laboratory conditions.
  • It is an in vitro process, unlike in vivo processes that occur inside the living body.
  • Cells are isolated from animal tissue and placed in a suitable medium to support survival and growth.
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2. Historical Development

  • Sydney Ringer developed a balanced salt solution in 1882 and kept frog hearts alive after dissection.
  • Ross Harrison published experiments in 1907 showing frog embryo nerve fibre growth in vitro.
  • Alexis Carrel introduced aseptic techniques to tissue culture in 1912 and used trypsin, embryo extracts, and animal serum.
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3. Culture Media and Supplements

  • A suitable growth medium is the most critical requirement for cell culture.
  • Culture media support cell survival, proliferation, pH balance, and osmolality.
  • Natural media consist of biological substances such as plasma, serum, and tissue extract.
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4. Physical Environment for Cell Growth

  • Animal cells require controlled temperature, pH, osmolality, gas mixture, and support surface.
  • CO2 incubators are used to grow mammalian cells at 37°C.
  • Typical mammalian cell culture conditions are 37°C, 95% humidity, and 5% CO2.

Key Concepts

Central concept: Animal cell culture as the in vitro maintenance and proliferation of animal cells outside the living organism under proper nutrients and growth conditions

Definition and basicsHistorical developmentCulture mediaPhysical environmentBuffering systems and indicatorsEquipment used for cultureTypes of animal cell cultureCell line life span

Frequently Asked Questions

What are the important topics in Animal Cell Culture for CBSE Class 12 Biotechnology?
Animal Cell Culture covers several key topics that are frequently asked in CBSE Class 12 board exams. Focus on the core concepts listed on this page and practise related questions to build confidence.
How to score full marks in Animal Cell Culture — CBSE Class 12 Biotechnology?
Understand the core concepts first, then work through the 77 practice questions available for this chapter. Revise formulas and definitions regularly, and use flashcards for quick recall before the exam.

Sources & Official References

Content is aligned to the official syllabus. Refer to the board website for the latest curriculum.

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All 13 chapters — CBSE Class 12 Biotechnology Syllabus

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