Genetics and Society — Flashcards
Telangana Open School (TOSS) · Class 12 · Biology
25 flashcards for Genetics and Society (Telangana Open School (TOSS) Class 12 Biology) to test yourself on key terms and facts.
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What is gene cloning?
Answer
Gene cloning is the process of producing multiple identical copies of a specific gene. This is done using recombinant DNA technology, where the desired gene is inserted into a bacterial plasmid. As th…
What is a gene bank?
Answer
A gene bank is a collection of bacterial or bacteriophage clones, each carrying a specific foreign DNA segment (gene). These clones are preserved at very low temperatures for future use. For example, …
Why are plasmids used in gene cloning?
Answer
Plasmids are small, circular DNA molecules found in bacteria that are separate from the bacterial chromosome. They are used as vectors in gene cloning because they can easily be removed, cut with rest…
What are restriction endonucleases and their role in rDNA technology?
Answer
Restriction endonucleases are enzymes that cut DNA at specific nucleotide sequences. They are used in recombinant DNA technology to cut both the desired gene from the source organism and the plasmid v…
Define recombinant DNA technology.
Answer
Recombinant DNA technology is a technique used to combine DNA from two different sources. It involves cutting a specific gene from one organism using restriction enzymes, inserting it into a plasmid v…
What is DNA splicing?
Answer
DNA splicing is the process of joining two pieces of DNA from different sources. In recombinant DNA technology, after cutting DNA with restriction enzymes, the desired gene is joined with a plasmid ve…
Explain the steps in recombinant DNA technology.
Answer
1. Isolation: The desired gene is cut from the source DNA using restriction enzymes. 2. Vector Preparation: A plasmid is cut with the same enzyme. 3. Recombination: The foreign gene is inserted into t…
What is a transgenic organism?
Answer
A transgenic organism is an organism that contains a foreign gene from another species in its genome. This is achieved through genetic engineering. For example, a transgenic goat may carry a human gen…
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